Isolation, Molecular identification of metal tolerant bacteria and its heavy metal removal capacity
The cadmium removal by isolated cadmium tolerant bacteria Pseudomonas sp isolated from electronic waste (e-waste) recycling facility soil was studied in a laboratory simulated environment. The bacterium was identified by biochemical and 16S rRNA molecular studies. The DNA was isolated and amplified using universal bacterial primers. The phylogeny tree suggested the isolated cadmium tolerant bacterium as Pseudomonas aeruginosa. The partial 16S rRNA sequences were submitted to NCBI Genbank and the accession number JN102340 was assigned to the submitted sequences. Various parameters were optimized for cadmium removal such as pH, temperature, kinetics studies and metal tolerant assay. The metal removal studies suggested that the cadmium tolerant Pseudomonas aeruginosa JN102340 able to remove cadmium 92% at pH 6, 89% at 35șC , 90% after 24h incubation in the optimized pH and temperature. The bacterium Pseudomonas aeruginosa JN102340 tolerate and survive up to 600mg L-1 cadmium. The abstract should not exceed 250 words, and should concisely summarize the basic content of the paper. Experimental details should not be presented in the abstract. Avoid specialized terms, abbreviations, diagrams, and references.