Computational Evaluation and Antimicrobial Effect of Boric Acid and Ascorbic Acid with Drug Resistant Clinical Pathogens
Thiyagarajamoorthy Dhinesh Kumar, Murugaiah Santhosh Gokul and Rathinam Arthur James
Abstract
Drug resistant sways are crossing its limits globally especially among the common clinical pathogens like E. coli, S. aureus etc. A world of antibiotics was created by the discovery of penicillin but at current scenario penicillin lost its potential effects due to the drug resistant mechanism of pathogens. A source of natural products like boric acid showed significant outcome against human clinical pathogens (HCP) such as S. aureus, S. typhi, E. coli, V. cholerae, S. epidermidis, and K. pneumoniae which was resistant to penicillin. In the preliminary part with 5mg concentration of 10µl boric acid was bared >10mm of zone of inhibition, ascorbic acid showed <10mm and with penicillin negative as a control. 10 µg Penicillin G as well as ascorbic acid lost their potency with in 12hrs against HCP but boric acid was stable up to 48hrs in the time of stability test. The toxicity analysis was done by VEGA and it was showed that boric acid was not act as mutagenic and carcinogenic agent. In the analysis LC50, 100mg/l was acceptable for boric acid and it was analysed by Fish acute (LC50) Toxicity classification (SarPy/IRFMN) 1.0.2.